Composite

Part:BBa_K2300000:Design

Designed by: Ari Edmonds   Group: iGEM17_Macquarie_Australia   (2017-10-03)


HydEFG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1849
    Illegal NheI site found at 2059
    Illegal NheI site found at 2503
    Illegal NotI site found at 253
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 901
    Illegal NgoMIV site found at 4857
    Illegal AgeI site found at 1816
    Illegal AgeI site found at 3459
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2684
    Illegal BsaI.rc site found at 304
    Illegal BsaI.rc site found at 416


Design Notes

Removal of introns and codon optimisation for E. coli.


Source

Chlamydomnas reinhardtii

References